SABIIA - Sistema Aberto e Integrado de Informação em Agricultura (Sabiia)

Chair, M.; Dehasque, M.; Van Poucke, S.; Nelis, H.; Sorgeloos, P.; De Leenheer, A.P..

There is a need for a standard in vitro procedure to infect larval fish with bacteria. Such a procedure could be used as a tool to evaluate fish quality, verify fish sensitivity to a certain bacterial strain or evaluate the pathogenicity of a bacterium towards a given fish species. The availability of a challenge test is a prerequisite in studies dealing with the effect of different medicinal treatments on diseased fish. An oral challenge test would offer the possibility to investigate the routes of infection via the digestive tract, i.e. after feeding contaminated feeds. The present study aims to verify this hypothesis and proposes a standard challenge procedure for turbot (Scophthalmus maximus) larvae with a pathogenic Vibrio...

Bacteries ichtyopathogenes en mariculture

Palavras-chave: Bacterial diseases; Digestive system; Disease control; Fish culture; Fish diseases; Fish larvae; Marine fish; Pathogenic bacteria; Vibrio anguillarum.

Ano: 1994

URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=2552

Comparative serology of the marine fish pathogen Vibrio anguillarum

Vigneulle, Martine.

Bacterial infections occurred during the development of marine fish farming of species such as turbot, sea-bass, sea-bream or salmon and trout. The main-specific bacteria involved in these diseases observed belong to 3 groups: the Vibrionaceae for Vibrio anguillarum (type 408) and Aeromonas salmonicida , the causative agents of respectively vibriosis and furinculosis; the Corynebacteriaceae for Renibacterium salmoninarum , responsible for bacterial kidney disease (BKD), the Enterobacteriaceae for Yersinia ruckeri , the causative agent of enteric redmouth disease. The clinical signs typical of those diseases always depend on the rearing and environmental conditions. The losses resulting from these bacterial diseases and the persistence of the bacteria in...

Tipo: Text

Palavras-chave: Yersinia ruckeri; Renibacterium salmoninarum; Aeromonas salmonicida; Vibrio anguillarum; Bacteria; Bacterial diseases; Fish culture; Pathogenic bacteria.

Ano: 1984

URL: http://archimer.ifremer.fr/doc/1984/acte-996.pdf

Grisez, L.; Ollevier, F.P..

The different serotyping systems, based on thermostable O antigens, reported for Vibrio anguillarum and V. ordalii were compared by quantitative agglutination, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and subsequent silver staining or Western blotting (immunoblotting) of purified lipopolysaccharide (LPS), using polyclonal rabbit antisera. The results demonstrate that 16 different serotypes within V. anguillarum (designated 01 to 016) can be distinguished. Each of these serotypes is characterized by a distinct polysaccharide banding pattern, as revealed by silver-stained gels of purified LPS. The comparative analysis allowed a complete alignment of the different stereotypes for the...

Effect of dietary water content on European sea bass (Dicentrarchus labrax) growth and disease resistance

Palavras-chave: Pathogens; Serology; Vibrio anguillarum.

Ano: 1995

URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=2970

Przybyla, Cyrille; Fievet, Julie; Callier, Myriam; Blancheton, Jean-paul.

The effect of dietary water content on Dicentrarchus labrax growth parameters and resistance to Vibrio anguillarum infection was investigated using commercial pellets with identical energy contents and different moisture levels. The first experiment hypothesis was that moisturizing pelleted ration can have an impact on Dicentrarchus labrax growth performance by the osmoregulation energy cost reduction. In a second time, the experiment explores the effect of water addition in pellets on the fish resistance to a disease. A specific device was built to uniformly moisturize dry pellets to different moisture levels, i.e. 8%, 20%, 40% and 60%. After an acclimation period and a 54-day rearing period, the control fish had grown from 72.7 +/- 17.9 g to 133.3 +/-...

Tipo: Text

Palavras-chave: Fish aquaculture; Dietary moisture content; Feed pellets; Fish growth; Immunity; Dicentrarchus labrax; Vibrio anguillarum.

Ano: 2014

URL: http://archimer.ifremer.fr/doc/00248/35927/34446.pdf

Etude ecologique des vibrions du bassin d'Arcachon

Extended serotyping scheme for Vibrio anguillarum with the definition and characterization of seven provisional O-serogroups

Marchand, Michel.

Water and sediment samples monthly collected from Arcachon Bay (Gironde, France) were examined during three years for the presence of marine vibrios. V. cholerae serotype 01 was never detected. On the other hand, the Eyre River and mouth contain non-agglutinating V. cholerae (127 strains) principally in water. The low temperatures slow their development down without stopping it completely. V. parahaemolyticus (257 strains) present in the same areas is particularly isolated from water when the aquatic medium temperature rises to 15 degree C. V. alginolyticus (638 strains) settles the whole Bay, except the river, in any season. The low temperatures promote growth of V. metschnikovii (203 strains) very tolerant towards salinity. V. anguillarum (46 strains)...

Tipo: Text

Palavras-chave: Vibrio metschnikovii; Vibrio anguillarum; Vibrio alginolyticus; Vibrio cholerae; Vibrio; Marine environment; Microorganisms; Sediments; Salinity effects; Temperature effects; Ecology; Water.

Ano: 1984

URL: http://archimer.ifremer.fr/doc/1984/acte-998.pdf

Pedersen, K.; Grisez, L.; van Houdt, R.; Tiainen, T.; Ollevier, F.P.; Larsen, J.L..

The present paper summarizes the serotyping scheme of the fish pathogenic bacterium Vibrio anguillarum and defines seven additional O-serogroups. Strains, collected in our laboratories that were nontypable with antisera against the previously defined 16 O-serotypes, were used for generating new antisera and were characterized further by means of LPS profiles, Western blots, and serological reactions. On the basis of the results, it is suggested that the seven new O-serogroups are to be included in the existing serotyping system as serotypes O17-O23. However, the existence of further V. anguillarum strains that were not typable with any of the 23 O-antisera suggested the existence of additional...

Lysozyme gene expression and hemocyte behaviour in the Mediterranean mussel, Mytilus galloprovincialis, after injection of various bacteria or temperature stresses

Palavras-chave: Bacterial diseases; Fish diseases; Pathogenic bacteria; Pathogens; Serological studies; Serotypes; Strains; Vibrio anguillarum.

Ano: 1999

URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=9525

The influence of a selected bacterial strain Vibrio anguillarum TR27 on the growth rate of the rotifer, Brachionus plicatilis in two culture conditions

Li, H; Parisi, M; Toubiana, Mylene; Cammarata, M; Roch, Philippe.

The aim of the present study was to evaluate the expression of the Mytilus galloprovincialis lysozyme gene in different in vivo stress situations, including injection of bacteria Vibrio splendidus LGP32, Vibrio anguillarum or Micrococcus lysodeikticus, as well as heat shock at 30 C and cold stress at 5 C. Injection of V. splendidus LGP32 resulted in: (i) a general down-regulation of lysozyme gene expression, as quantified by Q-PCR; (ii) reduction in the number of circulating hemocytes; (iii) decrease in the percentage of circulating hemocytes expressing lysozyme mRNA which was now restricted to only small cells, as observed by ISH; and (iv) accumulation of hemocytes expressing lysozyme in the muscle sinus where injection took place. Injection of V....

Tipo: Text

Palavras-chave: Molluscs; Mytilus; ISH; Q PCR; Gene expression; Lysozyme; Heat shock; Micrococcus lysodeikticus; Vibrio anguillarum; Vibrio splendidus.

Ano: 2008

URL: http://archimer.ifremer.fr/doc/2008/publication-4479.pdf

Shiri Harzevili, A.R.; Van Duffel, H.; Defoort, T.; Sorgeloos, P.; Swings, J..

The use of API 20E for the identification of Vibrio anguillarum and V. ordalii

Palavras-chave: Brachionus plicatilis; Vibrio anguillarum.

Ano: 1995

URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=2612

Grisez, L.; Ceusters, R.; Ollevier, F.P..

Sixty-eight strains of Vibrio anguillarum, five of V. ordalii and the type strains of V. alginolyticus, V. carchariae, V. damsela and V. parahaemolyticus were compared using the API 20E gallery. Within the V. anguillarum strains, distinct groups could be separated mainly on the basis of their reaction on indole production and the fermentation of amygdalin and arabinose. Vibrio ordalii, the former V. anguillarum biotope 2, could easily be separated from V. anguillarum and from the other fish pathogenic Vibrio spp.

Uptake of Vibrio anguillarum vaccine by Artemia salina as a potential oral delivery system to fish fry

Palavras-chave: Bacterial diseases; Disease control; Disease detection; Fish diseases; Identification; Vibrio carchariae; Vibrio ordalii; Vibrio alginolyticus; Vibrio anguillarum; Vibrio damsela; Vibrio parahaemolyticus.

Ano: 1991

URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=2859

Campbell, R.; Adams, A.; Tatner, M.F.; Chair, M.; Sorgeloos, P..

An ELISA to detect Vibrio anguillarum antigen in Artemia salina was developed. This assay was then used to determine the time course of antigen uptake by Artemia during incubations with Vibrio vaccine with a view to using Artemia as a vaccine carrier for oral vaccination of fish fry. The rate of uptake of vaccine by Artemia was shown to be concentration dependent. At a dose of 1.5x107 cfu ml-1, 1 mg wet weight of Artemia took up 1x105 cfu within 30min and reached maximum of 6x106 cfu after 60min. Thus each Artemia...